Protectin D1 is generated in asthma and dampens airway inflammation and hyperresponsiveness

BD Levy, P Kohli, K Gotlinger, O Haworth… - The Journal of …, 2007 - journals.aai.org
BD Levy, P Kohli, K Gotlinger, O Haworth, S Hong, S Kazani, E Israel, KJ Haley, CN Serhan
The Journal of Immunology, 2007journals.aai.org
Protectins are newly identified natural chemical mediators that counter leukocyte activation
to promote resolution of inflammation. In this study, we provide the first evidence for protectin
D1 (PD1, 10R, 17S-dihydroxy-docosa-4Z, 7Z, 11E, 13E, 15Z, 19Z-hexaenoic acid) formation
from docosahexaenoic acid in human asthma in vivo and PD1 counterregulatory actions in
allergic airway inflammation. PD1 and 17S-hydroxy-docosahexaenoic acid were present in
exhaled breath condensates from healthy subjects. Of interest, levels of PD1 were …
Abstract
Protectins are newly identified natural chemical mediators that counter leukocyte activation to promote resolution of inflammation. In this study, we provide the first evidence for protectin D1 (PD1, 10R, 17S-dihydroxy-docosa-4Z, 7Z, 11E, 13E, 15Z, 19Z-hexaenoic acid) formation from docosahexaenoic acid in human asthma in vivo and PD1 counterregulatory actions in allergic airway inflammation. PD1 and 17S-hydroxy-docosahexaenoic acid were present in exhaled breath condensates from healthy subjects. Of interest, levels of PD1 were significantly lower in exhaled breath condensates from subjects with asthma exacerbations. PD1 was also present in extracts of murine lungs from both control animals and those sensitized and aerosol challenged with allergen. When PD1 was administered before aeroallergen challenge, airway eosinophil and T lymphocyte recruitment were decreased, as were airway mucus, levels of specific proinflammatory mediators, including IL-13, cysteinyl leukotrienes, and PGD 2, and airway hyperresponsiveness to inhaled methacholine. Of interest, PD1 treatment after aeroallergen challenge markedly accelerated the resolution of airway inflammation. Together, these findings provide evidence for endogenous PD1 as a pivotal counterregulatory signal in allergic airway inflammation and point to new therapeutic strategies for modulating inflammation in asthmatic lung.
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