Carboxy-terminal amino acid sequence of a human fibrinogen. gamma.-chain variant (. gamma.')
C Wolfenstein-Todel, MW Mosesson - Biochemistry, 1981 - ACS Publications
C Wolfenstein-Todel, MW Mosesson
Biochemistry, 1981•ACS PublicationsMaterials and Methods Plasma fibrinogen fraction 1-4 (Blomback & Blomback,
1956)(thrombin clottability> 95%), the 7-chain population of which is comprised of
approximately7-10% y'chains (Mosesson et al., 1972), was prepared as previously
described (Mosesson & Sherry, 1966). Reduction and S-carboxy-methylation were carried
out as previously reported (Galanakis et al., 1978), and the S-CM1 7 and y'chains were
isolated by DEAE-cellulose gradient elution chromatography in 8 M urea at pH 7.0 …
1956)(thrombin clottability> 95%), the 7-chain population of which is comprised of
approximately7-10% y'chains (Mosesson et al., 1972), was prepared as previously
described (Mosesson & Sherry, 1966). Reduction and S-carboxy-methylation were carried
out as previously reported (Galanakis et al., 1978), and the S-CM1 7 and y'chains were
isolated by DEAE-cellulose gradient elution chromatography in 8 M urea at pH 7.0 …
Materials and Methods
Plasma fibrinogen fraction 1-4 (Blomback & Blomback, 1956)(thrombin clottability> 95%), the 7-chain population of which is comprised of approximately7-10% y'chains (Mosesson et al., 1972), was prepared as previously described (Mosesson & Sherry, 1966). Reduction and S-carboxy-methylation were carried out as previously reported (Galanakis et al., 1978), and the S-CM1 7 and y'chains were isolated by DEAE-cellulose gradient elution chromatography in 8 M urea at pH 7.0 (Stathakis et al., 1978). CNBr cleavage of S-CM peptides was carried out as described by Gross &
ACS Publications