Sensitivity to chemical oxidants and radiation in CHO cell lines deficient in oxidative pentose cycle activity
SW Tuttle, ME Varnes, JB Mitchell… - International Journal of …, 1992 - Elsevier
In this paper we examine the susceptibility of a series of G6PD− CHO cell lines to a variety of
chemical oxidants. Addition of these drugs to KID, the parental cell line, results in as much
as a 20-fold increase in pentose cycle (PC) activity over control values. In two of our mutant
lines, E16 and E48, little or no stimulation of PC activity is seen. These lines are shown to be
much more susceptible to the toxic effects of the chemical oxidants t-butyl hydroperoxide
and diamide. PC activity is also stimulated by ionizing radiation in KID cells. One of the …
chemical oxidants. Addition of these drugs to KID, the parental cell line, results in as much
as a 20-fold increase in pentose cycle (PC) activity over control values. In two of our mutant
lines, E16 and E48, little or no stimulation of PC activity is seen. These lines are shown to be
much more susceptible to the toxic effects of the chemical oxidants t-butyl hydroperoxide
and diamide. PC activity is also stimulated by ionizing radiation in KID cells. One of the …