[HTML][HTML] Glutathione and glutaredoxin act as a backup of human thioredoxin reductase 1 to reduce thioredoxin 1 preventing cell death by aurothioglucose
Y Du, H Zhang, J Lu, A Holmgren - Journal of Biological Chemistry, 2012 - ASBMB
Thioredoxin reductase 1 (TrxR1) in cytosol is the only known reductant of oxidized
thioredoxin 1 (Trx1) in vivo so far. We and others found that aurothioglucose (ATG), a well
known active-site inhibitor of TrxR1, inhibited TrxR1 activity in HeLa cell cytosol but had no
effect on the viability of the cells. Using a redox Western blot analysis, no change was
observed in redox state of Trx1, which was mainly fully reduced with five sulfhydryl groups.
In contrast, auranofin killed cells and oxidized Trx1, also targeting mitochondrial TrxR2 and …
thioredoxin 1 (Trx1) in vivo so far. We and others found that aurothioglucose (ATG), a well
known active-site inhibitor of TrxR1, inhibited TrxR1 activity in HeLa cell cytosol but had no
effect on the viability of the cells. Using a redox Western blot analysis, no change was
observed in redox state of Trx1, which was mainly fully reduced with five sulfhydryl groups.
In contrast, auranofin killed cells and oxidized Trx1, also targeting mitochondrial TrxR2 and …