A novel internally controlled real-time reverse transcription-PCR assay for HIV-1 RNA targeting the pol integrase genomic region
J Müller, AM Eis-Hübinger, M Däumer, R Kaiser… - Journal of virological …, 2007 - Elsevier
Given the worldwide increasing spread of HIV-1 genetic variants, it is mandatory that assays
used for nucleic acid testing for HIV-1 detect all existing groups and subtypes of HIV-1. In
this report the development and evaluation of a quantitative real-time HIV-1 RT-PCR assay
that targets a conserved region within the pol integrase domain is described. As an internal
control reaction, endogenous glyceraldehyde-3-phosphate-dehydrogenase transcripts were
detected in a multiplex configuration. The detection limit (95% cut-off value) was determined …
used for nucleic acid testing for HIV-1 detect all existing groups and subtypes of HIV-1. In
this report the development and evaluation of a quantitative real-time HIV-1 RT-PCR assay
that targets a conserved region within the pol integrase domain is described. As an internal
control reaction, endogenous glyceraldehyde-3-phosphate-dehydrogenase transcripts were
detected in a multiplex configuration. The detection limit (95% cut-off value) was determined …