[HTML][HTML] Hepatitis B surface antigen on subviral particles reduces the neutralizing effect of anti-HBs antibodies on hepatitis B viral particles in vitro
During hepatitis B virus (HBV) infections subviral particles (SVP) consisting mainly of
hepatitis B surface antigen are present at much higher concentration than viral particles (VP)
in serum. To investigate reasons for this excess of SVP production, SVP and VP were
fractionated on a Nycodenz gradient and analyzed for HBV infection of HepG2-NTCP cells
with and without anti-HBs antibodies. Our findings showed that SVP significantly reduced
the neutralization of VP by anti-HBs, while SVP had little effect on viral entry, supporting the …
hepatitis B surface antigen are present at much higher concentration than viral particles (VP)
in serum. To investigate reasons for this excess of SVP production, SVP and VP were
fractionated on a Nycodenz gradient and analyzed for HBV infection of HepG2-NTCP cells
with and without anti-HBs antibodies. Our findings showed that SVP significantly reduced
the neutralization of VP by anti-HBs, while SVP had little effect on viral entry, supporting the …
Abstract
During hepatitis B virus (HBV) infections subviral particles (SVP) consisting mainly of hepatitis B surface antigen are present at much higher concentration than viral particles (VP) in serum. To investigate reasons for this excess of SVP production, SVP and VP were fractionated on a Nycodenz gradient and analyzed for HBV infection of HepG2-NTCP cells with and without anti-HBs antibodies. Our findings showed that SVP significantly reduced the neutralization of VP by anti-HBs, while SVP had little effect on viral entry, supporting the assumption that SVP serve as decoy facilitating cell-to-cell spread of HBV in the presence of neutralizing antibodies.
Elsevier