Direct ex vivo evaluation of long-lived protective antiviral memory B cell responses against hepatitis B virus
SM Ward, P Phalora, D Bradshaw… - The Journal of …, 2008 - academic.oup.com
The Journal of infectious diseases, 2008•academic.oup.com
Background. The frequency of protective antiviral memory B cells after hepatitis B virus
(HBV) vaccination is unknown. Methods. A novel 2-step immunomagnetic protocol to assess
the ex vivo frequency of protective HBV surface antigen (HBsAg)—specific memory B cells
was used. Results. HBsAg-specific memory B cells were detected in vaccinated individuals,
although at very low frequency (median, 0.2% of CD19+ cells [range, 0%–4% of CD19+
cells]). No correlation existed between the frequency of HBsAg-specific memory B cells and …
(HBV) vaccination is unknown. Methods. A novel 2-step immunomagnetic protocol to assess
the ex vivo frequency of protective HBV surface antigen (HBsAg)—specific memory B cells
was used. Results. HBsAg-specific memory B cells were detected in vaccinated individuals,
although at very low frequency (median, 0.2% of CD19+ cells [range, 0%–4% of CD19+
cells]). No correlation existed between the frequency of HBsAg-specific memory B cells and …
Abstract
Background. The frequency of protective antiviral memory B cells after hepatitis B virus (HBV) vaccination is unknown.
Methods. A novel 2-step immunomagnetic protocol to assess the ex vivo frequency of protective HBV surface antigen (HBsAg)—specific memory B cells was used.
Results. HBsAg-specific memory B cells were detected in vaccinated individuals, although at very low frequency (median, 0.2% of CD19+ cells [range, 0%–4% of CD19+ cells]). No correlation existed between the frequency of HBsAg-specific memory B cells and the corresponding serum antibody titer or B cell enzyme-linked immunosorbent spot findings.
Conclusion. Our results indicate sustained B cell—mediated protection against HBV despite waning antibody titers, which is consistent with clinical observations.
Oxford University Press