[HTML][HTML] Kinetics of α-globin binding to α-hemoglobin stabilizing protein (AHSP) indicate preferential stabilization of hemichrome folding intermediate
Human α-hemoglobin stabilizing protein (AHSP) is a conserved mammalian erythroid
protein that facilitates the production of Hemoglobin A by stabilizing free α-globin. AHSP
rapidly binds to ferrous α with association (k′ AHSP) and dissociation (k AHSP) rate
constants of≈ 10 μm− 1 s− 1 and 0.2 s− 1, respectively, at pH 7.4 at 22° C. A small slow
phase was observed when AHSP binds to excess ferrous αCO. This slow phase appears to
be due to cis to trans prolyl isomerization of the Asp 29-Pro 30 peptide bond in wild-type …
protein that facilitates the production of Hemoglobin A by stabilizing free α-globin. AHSP
rapidly binds to ferrous α with association (k′ AHSP) and dissociation (k AHSP) rate
constants of≈ 10 μm− 1 s− 1 and 0.2 s− 1, respectively, at pH 7.4 at 22° C. A small slow
phase was observed when AHSP binds to excess ferrous αCO. This slow phase appears to
be due to cis to trans prolyl isomerization of the Asp 29-Pro 30 peptide bond in wild-type …