Automated quantitative analysis to assess motor function in different rat models of impaired coordination and ataxia
EI Kyriakou, JG van der Kieft, RC de Heer… - Journal of Neuroscience …, 2016 - Elsevier
Journal of Neuroscience Methods, 2016•Elsevier
Background An objective and automated method for assessing alterations in gait and motor
coordination in different animal models is important for proper gait analysis. The CatWalk
system has been used in pain research, ischemia, arthritis, spinal cord injury and some
animal models for neurodegenerative diseases. New method Our goals were to obtain a
comprehensive gait analysis of three different rat models and to identify which motor
coordination parameters are affected and are the most suitable and sensitive to describe …
coordination in different animal models is important for proper gait analysis. The CatWalk
system has been used in pain research, ischemia, arthritis, spinal cord injury and some
animal models for neurodegenerative diseases. New method Our goals were to obtain a
comprehensive gait analysis of three different rat models and to identify which motor
coordination parameters are affected and are the most suitable and sensitive to describe …
Background
An objective and automated method for assessing alterations in gait and motor coordination in different animal models is important for proper gait analysis. The CatWalk system has been used in pain research, ischemia, arthritis, spinal cord injury and some animal models for neurodegenerative diseases.
New method
Our goals were to obtain a comprehensive gait analysis of three different rat models and to identify which motor coordination parameters are affected and are the most suitable and sensitive to describe and detect ataxia with a secondary focus on possible training effects.
Results
Both static and dynamic parameters showed significant differences in all three models: enriched housed rats show higher walking and swing speed and longer stride length, ethanol-induced ataxia affects mainly the hind part of the body, and the SCA17 rats show coordination disturbances. Coordination changes were revealed only in the case of the ethanol-induced ataxia and the SCA17 rat model. Although training affected some gait parameters, it did not obscure group differences when those were present.
Comparison with existing methods
To our knowledge, a comparative gait assessment in rats with enriched housing conditions, ethanol-induced ataxia and SCA17 has not been presented before.
Conclusions
There is no gold standard for the use of CatWalk. Dependent on the specific effects expected, the protocol can be adjusted. By including all sessions in the analysis, any training effect should be detectable and the development of the performance over the sessions can provide insight in effects attributed to intervention, treatment or injury.
Elsevier