[HTML][HTML] Thermodynamics of oxidation-reduction reactions in mammalian nitric-oxide synthase isoforms
YT Gao, SME Smith, JB Weinberg… - Journal of Biological …, 2004 - ASBMB
The three mammalian nitric-oxide synthases produce NO from arginine in a reaction
requiring 3 electrons per NO, which are supplied to the catalytic center from NADPH through
reductase domains incorporating FAD and FMN cofactors. The isoforms share a common
reaction mechanism and requirements for reducing equivalents but differ in regulation; the
endothelial and neuronal isoforms are controlled by calcium/calmodulin modulation of the
electron transfer system, while the inducible isoform binds calmodulin at all physiological Ca …
requiring 3 electrons per NO, which are supplied to the catalytic center from NADPH through
reductase domains incorporating FAD and FMN cofactors. The isoforms share a common
reaction mechanism and requirements for reducing equivalents but differ in regulation; the
endothelial and neuronal isoforms are controlled by calcium/calmodulin modulation of the
electron transfer system, while the inducible isoform binds calmodulin at all physiological Ca …