Flexible Accelerated STOP Tetracycline Operator-knockin (FAST): a versatile and efficient new gene modulating system
We created the Flexible Accelerated STOP Tetracycline Operator (tetO)-knockin (FAST)
system, an efficient method for manipulating gene expression in vivo to rapidly screen
animal models of disease. A single gene targeting event yields two distinct knockin mice—
STOP-tetO and tetO knockin—that permit generation of multiple strains with variable
expression patterns: 1) knockout, 2) Cre-mediated rescue, 3) tetracycline-controlled
transcriptional activator (tTA)-mediated misexpression, 4) tetracycline-controlled …
system, an efficient method for manipulating gene expression in vivo to rapidly screen
animal models of disease. A single gene targeting event yields two distinct knockin mice—
STOP-tetO and tetO knockin—that permit generation of multiple strains with variable
expression patterns: 1) knockout, 2) Cre-mediated rescue, 3) tetracycline-controlled
transcriptional activator (tTA)-mediated misexpression, 4) tetracycline-controlled …