An organotypic high throughput system for characterization of drug sensitivity of primary multiple myeloma cells
A Silva, T Jacobson, M Meads, A Distler… - JoVE (Journal of …, 2015 - jove.com
In this work we describe a novel approach that combines ex vivo drug sensitivity assays and
digital image analysis to estimate chemosensitivity and heterogeneity of patient-derived
multiple myeloma (MM) cells. This approach consists in seeding primary MM cells freshly
extracted from bone marrow aspirates into microfluidic chambers implemented in multi-well
plates, each consisting of a reconstruction of the bone marrow microenvironment, including
extracellular matrix (collagen or basement membrane matrix) and stroma (patient-derived …
digital image analysis to estimate chemosensitivity and heterogeneity of patient-derived
multiple myeloma (MM) cells. This approach consists in seeding primary MM cells freshly
extracted from bone marrow aspirates into microfluidic chambers implemented in multi-well
plates, each consisting of a reconstruction of the bone marrow microenvironment, including
extracellular matrix (collagen or basement membrane matrix) and stroma (patient-derived …