Class II MHC antigen expression by cultured human cerebral vascular endothelial cells

RM McCarron, L Wang, EP Cowan, M Spatz - Brain research, 1991 - Elsevier
RM McCarron, L Wang, EP Cowan, M Spatz
Brain research, 1991Elsevier
Cerebral vascular endothelial cells (EC) isolated from human brain do not constitutively
express class II MHC antigens. However, incubation in the presence of human interferon-
gamma (IFNγ) resulted in the expression of both HLA-DR and-DP antigens. FACS analysis
revealed that approximately 40% of the EC population expressed HLA-DR antigen.
Quantitation by ELISA demonstrated that maximum expression was observed with 100 U/ml
IFNγ for 4 days. Treatment with IFNγ also increased class II mRNA levels in all EC cultures …
Abstract
Cerebral vascular endothelial cells (EC) isolated from human brain do not constitutively express class II MHC antigens. However, incubation in the presence of human interferon-gamma (IFNγ) resulted in the expression of both HLA-DR and -DP antigens. FACS analysis revealed that approximately 40% of the EC population expressed HLA-DR antigen. Quantitation by ELISA demonstrated that maximum expression was observed with 100 U/ml IFNγ for 4 days. Treatment with IFNγ also increased class II mRNA levels in all EC cultures tested.
Elsevier