Establishment and characterization of a novel myeloid cell line from the bone marrow of a patient with the myelodysplastic syndrome
K Tohyama, H Tsutani, T Ueda… - British journal of …, 1994 - Wiley Online Library
K Tohyama, H Tsutani, T Ueda, T Nakamura, Y Yoshida
British journal of haematology, 1994•Wiley Online LibraryA novel long‐term cultured myeloid cell line was established from the bone marrow of a
patient with myelodysplastic syndrome (MDS). This cell line, designated MDS92. proliferated
in the presence of interleukin‐3 or granulocyte‐macrophage colony‐stimulating factor and
transiently in the presence of Steel factor, with a tendency for gradual maturation, and
formed myeloid colonies in the semi‐solid culture condition. In addition, the MDS92 cell line
represented rather complicated karyotypic abnormalities including the deletion of fifth and …
patient with myelodysplastic syndrome (MDS). This cell line, designated MDS92. proliferated
in the presence of interleukin‐3 or granulocyte‐macrophage colony‐stimulating factor and
transiently in the presence of Steel factor, with a tendency for gradual maturation, and
formed myeloid colonies in the semi‐solid culture condition. In addition, the MDS92 cell line
represented rather complicated karyotypic abnormalities including the deletion of fifth and …
Summary
A novel long‐term cultured myeloid cell line was established from the bone marrow of a patient with myelodysplastic syndrome (MDS). This cell line, designated MDS92. proliferated in the presence of interleukin‐3 or granulocyte‐macrophage colony‐stimulating factor and transiently in the presence of Steel factor, with a tendency for gradual maturation, and formed myeloid colonies in the semi‐solid culture condition.
In addition, the MDS92 cell line represented rather complicated karyotypic abnormalities including the deletion of fifth and seventh chromosomes and a point mutation at codon 12 of the N‐ras oncogene.
These characteristics of the MDS92 cell line are exclusively compatible with the property of preleukaemia.
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