Lipofuscin is formed independently of macroautophagy and lysosomal activity in stress-induced prematurely senescent human fibroblasts
A Höhn, A Sittig, T Jung, S Grimm, T Grune - Free Radical Biology and …, 2012 - Elsevier
A Höhn, A Sittig, T Jung, S Grimm, T Grune
Free Radical Biology and Medicine, 2012•ElsevierIn the current literature, the lysosomal system is considered to be involved in the intracellular
formation and accumulation of lipofuscin, a highly oxidized and covalently cross-linked
aggregate of proteins that fills the lysosomal volume during aging. In contrast, our
experimental results presented here suggest that both the autophagosomes and the
lysosomal system are not mandatory for the formation of lipofuscin, since that material
accumulates in the cytosolic volume if autophagy or lysosomal activity is inhibited. However …
formation and accumulation of lipofuscin, a highly oxidized and covalently cross-linked
aggregate of proteins that fills the lysosomal volume during aging. In contrast, our
experimental results presented here suggest that both the autophagosomes and the
lysosomal system are not mandatory for the formation of lipofuscin, since that material
accumulates in the cytosolic volume if autophagy or lysosomal activity is inhibited. However …
In the current literature, the lysosomal system is considered to be involved in the intracellular formation and accumulation of lipofuscin, a highly oxidized and covalently cross-linked aggregate of proteins that fills the lysosomal volume during aging. In contrast, our experimental results presented here suggest that both the autophagosomes and the lysosomal system are not mandatory for the formation of lipofuscin, since that material accumulates in the cytosolic volume if autophagy or lysosomal activity is inhibited. However, such an inhibition is accompanied by an enhanced toxicity of the formed protein aggregates. Furthermore, it could be proven that macroautophagy is responsible for the uptake of lipofuscin into the lysosomes.
Elsevier