Transduction of an HLA-DP4–restricted NY-ESO-1–specific TCR into Primary Human CD4+ Lymphocytes

Y Zhao, Z Zheng, HT Khong… - Journal of …, 2006 - journals.lww.com
Abstract cDNAs encoding functional T cell receptor (TCR) α and β chains from a CD4+ T cell
line (SG6) generated by repeated stimulation of a melanoma patient's peripheral blood
mononuclear cells with HLA-DP4–restricted, NY-ESO-1–specific peptide p161-180 were
cloned using a 5′ rapid amplification of cDNA end method. Three different TCR α chains
and 7 TCR β chains were found among the 84 α and 162 β cDNA clones tested. By
screening different combination of the α/β chains using RNA electroporation, TRAV9-1 …