A public genome-scale lentiviral expression library of human ORFs

X Yang, JS Boehm, X Yang, K Salehi-Ashtiani, T Hao… - Nature …, 2011 - nature.com
X Yang, JS Boehm, X Yang, K Salehi-Ashtiani, T Hao, Y Shen, R Lubonja, SR Thomas…
Nature methods, 2011nature.com
Functional characterization of the human genome requires tools for systematically
modulating gene expression in both loss-of-function and gain-of-function experiments. We
describe the production of a sequence-confirmed, clonal collection of over 16,100 human
open-reading frames (ORFs) encoded in a versatile Gateway vector system. Using this
ORFeome resource, we created a genome-scale expression collection in a lentiviral vector,
thereby enabling both targeted experiments and high-throughput screens in diverse cell …
Abstract
Functional characterization of the human genome requires tools for systematically modulating gene expression in both loss-of-function and gain-of-function experiments. We describe the production of a sequence-confirmed, clonal collection of over 16,100 human open-reading frames (ORFs) encoded in a versatile Gateway vector system. Using this ORFeome resource, we created a genome-scale expression collection in a lentiviral vector, thereby enabling both targeted experiments and high-throughput screens in diverse cell types.
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