Sequence specificity of the core-binding factor
IN Melnikova, BE Crute, S Wang, NA Speck - Journal of virology, 1993 - Am Soc Microbiol
IN Melnikova, BE Crute, S Wang, NA Speck
Journal of virology, 1993•Am Soc MicrobiolThe core-binding factor (CBF) binds the conserved core motif in mammalian type C
retrovirus enhancers. We analyzed the phosphate contacts made by CBF on the Moloney
murine leukemia virus enhancer by ethylation interference assay. The phosphate contacts
span 9 bp centered around the consensus core site. To examine the sequence preferences
for CBF binding, we employed the technique of selected and amplified binding sequence
footprinting (TK Blackwell and H. Weintraub, Science 250: 1104-1110, 1990). The …
retrovirus enhancers. We analyzed the phosphate contacts made by CBF on the Moloney
murine leukemia virus enhancer by ethylation interference assay. The phosphate contacts
span 9 bp centered around the consensus core site. To examine the sequence preferences
for CBF binding, we employed the technique of selected and amplified binding sequence
footprinting (TK Blackwell and H. Weintraub, Science 250: 1104-1110, 1990). The …
The core-binding factor (CBF) binds the conserved core motif in mammalian type C retrovirus enhancers. We analyzed the phosphate contacts made by CBF on the Moloney murine leukemia virus enhancer by ethylation interference assay. The phosphate contacts span 9 bp centered around the consensus core site. To examine the sequence preferences for CBF binding, we employed the technique of selected and amplified binding sequence footprinting (T. K. Blackwell and H. Weintraub, Science 250:1104-1110, 1990). The consensus binding site for CBF defined by selected and amplified binding sequence footprinting is PyGPyG GTPy.
American Society for Microbiology