Structure of a complex between a voltage-gated calcium channel β-subunit and an α-subunit domain

F Van Petegem, KA Clark, FC Chatelain, DL Minor Jr - Nature, 2004 - nature.com
F Van Petegem, KA Clark, FC Chatelain, DL Minor Jr
Nature, 2004nature.com
Voltage-gated calcium channels (CaVs) govern muscle contraction, hormone and
neurotransmitter release, neuronal migration, activation of calcium-dependent signalling
cascades, and synaptic input integration. An essential CaV intracellular protein, the β-
subunit (CaVβ),, binds a conserved domain (the α-interaction domain, AID) between
transmembrane domains I and II of the pore-forming α1 subunit and profoundly affects
multiple channel properties such as voltage-dependent activation, inactivation rates, G …
Abstract
Voltage-gated calcium channels (CaVs) govern muscle contraction, hormone and neurotransmitter release, neuronal migration, activation of calcium-dependent signalling cascades, and synaptic input integration. An essential CaV intracellular protein, the β-subunit (CaVβ),, binds a conserved domain (the α-interaction domain, AID) between transmembrane domains I and II of the pore-forming α1 subunit and profoundly affects multiple channel properties such as voltage-dependent activation, inactivation rates, G-protein modulation, drug sensitivity and cell surface expression,. Here, we report the high-resolution crystal structures of the CaVβ2a conserved core, alone and in complex with the AID. Previous work suggested that a conserved region, the β-interaction domain (BID), formed the AID-binding site,; however, this region is largely buried in the CaVβ core and is unavailable for protein–protein interactions. The structure of the AID–CaVβ2a complex shows instead that CaVβ2a engages the AID through an extensive, conserved hydrophobic cleft (named the α-binding pocket, ABP). The ABP–AID interaction positions one end of the CaVβ near the intracellular end of a pore-lining segment, called IS6, that has a critical role in CaV inactivation,. Together, these data suggest that CaVβs influence CaV gating by direct modulation of IS6 movement within the channel pore.
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