[HTML][HTML] Involvement of dihydroceramide desaturase in cell cycle progression in human neuroblastoma cells

JM Kraveka, L Li, ZM Szulc, J Bielawski… - Journal of Biological …, 2007 - ASBMB
The role of dihydroceramide desaturase as a key enzyme in the de novo pathway of
ceramide generation was investigated in human neuroblastoma cells (SMS-KCNR). A novel
assay using water-soluble analogs of dihydroceramide, dihydroceramidoids (d-erythro-
dhCCPS analogs), was used to measure desaturase activity in situ. Conversion of d-erythro-
2-N-[12′-(1 ″-pyridinium)-dodecanoyl]-4, 5-dihydrosphingosine bromide (C 12-dhCCPS)
to its 4, 5-desaturated counterpart, d-erythro-2-N-[12′-(1 ″-pyridinium) dodecanoyl] …