Costimulation of T cells by B7-H2, a B7-like molecule that binds ICOS
S Wang, G Zhu, AI Chapoval, H Dong… - Blood, The Journal …, 2000 - ashpublications.org
Blood, The Journal of the American Society of Hematology, 2000•ashpublications.org
This report describes a new human B7-like gene designated B7-H2. Cell surface expression
of B7-H2 protein is detected in monocyte-derived immature dendritic cells. Soluble B7-H2
and immunoglobulin (Ig) fusion protein, B7-H2Ig, binds activated but not resting T cells and
the binding is abrogated by inducible costimulator Ig (ICOSIg), but not CTLA4Ig. In addition,
ICOSIg stains Chinese hamster ovary cells transfected with B7-H2 gene. By suboptimal
cross-linking of CD3, costimulation of T-cell proliferation by B7-H2Ig is dose-dependent and …
of B7-H2 protein is detected in monocyte-derived immature dendritic cells. Soluble B7-H2
and immunoglobulin (Ig) fusion protein, B7-H2Ig, binds activated but not resting T cells and
the binding is abrogated by inducible costimulator Ig (ICOSIg), but not CTLA4Ig. In addition,
ICOSIg stains Chinese hamster ovary cells transfected with B7-H2 gene. By suboptimal
cross-linking of CD3, costimulation of T-cell proliferation by B7-H2Ig is dose-dependent and …
Abstract
This report describes a new human B7-like gene designatedB7-H2. Cell surface expression of B7-H2 protein is detected in monocyte-derived immature dendritic cells. Soluble B7-H2 and immunoglobulin (Ig) fusion protein, B7-H2Ig, binds activated but not resting T cells and the binding is abrogated by inducible costimulator Ig (ICOSIg), but not CTLA4Ig. In addition, ICOSIg stains Chinese hamster ovary cells transfected with B7-H2 gene. By suboptimal cross-linking of CD3, costimulation of T-cell proliferation by B7-H2Ig is dose-dependent and correlates with secretion of interleukin (IL)-2, whereas optimal CD3 ligation preferentially stimulates IL-10 production. The results indicate that B7-H2 is a putative ligand for the ICOS T-cell molecule.
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