Measurement of epidermal growth factor receptor turnover and effects of Cdc42
Q Lin, W Yang, RA Cerione - Methods in enzymology, 2006 - Elsevier
Ligand‐induced degradation represents an essential component of the overall regulation of
EGF receptor (EGFR)–coupled signal transduction. Following activation, EGFRs are
monoubiquitinated, subsequently sorted by ubiquitin‐interaction–based sorting machinery,
and transported to multivesicular bodies (MVBs) and lysosomes for degradation. The Rho‐
family small G‐protein, Cdc42, has been implicated in the regulation of EGFR degradation.
Here we describe routine methods for assaying EGFR endocytosis and degradation. In …
EGF receptor (EGFR)–coupled signal transduction. Following activation, EGFRs are
monoubiquitinated, subsequently sorted by ubiquitin‐interaction–based sorting machinery,
and transported to multivesicular bodies (MVBs) and lysosomes for degradation. The Rho‐
family small G‐protein, Cdc42, has been implicated in the regulation of EGFR degradation.
Here we describe routine methods for assaying EGFR endocytosis and degradation. In …