[HTML][HTML] Oral tolerance in the absence of naturally occurring Tregs

D Mucida, N Kutchukhidze, A Erazo… - The Journal of …, 2005 - Am Soc Clin Investig
D Mucida, N Kutchukhidze, A Erazo, M Russo, JJ Lafaille, MAC de Lafaille
The Journal of clinical investigation, 2005Am Soc Clin Investig
Mucosal tolerance prevents pathological reactions against environmental and food
antigens, and its failure results in exacerbated inflammation typical of allergies and asthma.
One of the proposed mechanisms of oral tolerance is the induction of Tregs. Using a mouse
model of hyper-IgE and asthma, we found that oral tolerance could be effectively induced in
the absence of naturally occurring thymus-derived Tregs. Oral antigen administration prior to
ip immunization prevented effector/memory Th2 cell development, germinal center …
Mucosal tolerance prevents pathological reactions against environmental and food antigens, and its failure results in exacerbated inflammation typical of allergies and asthma. One of the proposed mechanisms of oral tolerance is the induction of Tregs. Using a mouse model of hyper-IgE and asthma, we found that oral tolerance could be effectively induced in the absence of naturally occurring thymus-derived Tregs. Oral antigen administration prior to i.p. immunization prevented effector/memory Th2 cell development, germinal center formation, class switching to IgE, and lung inflammation. Oral exposure to antigen induced development of antigen-specific CD4+CD25+Foxp3+CD45RBlow cells that were anergic and displayed suppressive activity in vivo and in vitro. Oral tolerance to the Th2 allergic response was in large part dependent on TGF-β and independent of IL-10. Interestingly, Tregs were also induced by single i.p. immunization with antigen and adjuvant. However, unlike oral administration of antigen, which induced Tregs but not effector T cells, i.p. immunization led to the simultaneous induction of Tregs and effector Th2 cells displaying the same antigen specificity.
The Journal of Clinical Investigation