[HTML][HTML] Live Cell Analysis of Aquaporin-4 M1/M23 Interactions and Regulated Orthogonal Array Assembly in Glial Cells*♦
JM Crane, JL Bennett, AS Verkman - Journal of biological chemistry, 2009 - ASBMB
Aquaporin-4 (AQP4) can assemble into supramolecular aggregates called orthogonal
arrays of particles (OAPs). In cells expressing single AQP4 isoforms, we found previously
that OAP formation by AQP4-M23 requires N terminus interactions just downstream of Met-
23 and that the inability of AQP4-M1 to form OAPs involves blocking by residues upstream of
Met-23. Here, we studied M1/M23 interactions and regulated OAP assembly by nanometer-
resolution tracking of quantum dot-labeled AQP4 in live cells expressing differentially tagged …
arrays of particles (OAPs). In cells expressing single AQP4 isoforms, we found previously
that OAP formation by AQP4-M23 requires N terminus interactions just downstream of Met-
23 and that the inability of AQP4-M1 to form OAPs involves blocking by residues upstream of
Met-23. Here, we studied M1/M23 interactions and regulated OAP assembly by nanometer-
resolution tracking of quantum dot-labeled AQP4 in live cells expressing differentially tagged …