A method for intratracheal instillation of endotoxin into the lungs of mice
B Starcher, I Williams - Laboratory animals, 1989 - journals.sagepub.com
B Starcher, I Williams
Laboratory animals, 1989•journals.sagepub.comA detailed method is presented for the intratracheal instillation of fluids into the lungs of
mice. Using 125I-albumen it was determined that 25-50 µ l of volume gave almost equal
distribution to all lobes of the mouse lung. Extremely low levels of endotoxin given
intratracheally (12· 5-25 ng) resulted in a dramatic increase in the neutrophil content of the
lung. The increase started in less than 4 h, reaching levels of 7× 105 by 18 h and was
maintained at elevated levels for at least 48 h. This neutrophil count represented more than …
mice. Using 125I-albumen it was determined that 25-50 µ l of volume gave almost equal
distribution to all lobes of the mouse lung. Extremely low levels of endotoxin given
intratracheally (12· 5-25 ng) resulted in a dramatic increase in the neutrophil content of the
lung. The increase started in less than 4 h, reaching levels of 7× 105 by 18 h and was
maintained at elevated levels for at least 48 h. This neutrophil count represented more than …
A detailed method is presented for the intratracheal instillation of fluids into the lungs of mice. Using 125I-albumen it was determined that 25-50 µl of volume gave almost equal distribution to all lobes of the mouse lung. Extremely low levels of endotoxin given intratracheally (12·5-25 ng) resulted in a dramatic increase in the neutrophil content of the lung. The increase started in less than 4 h, reaching levels of 7 × 105 by 18 h and was maintained at elevated levels for at least 48 h. This neutrophil count represented more than 90% of the cell population of the lung.
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