Isolation of human periosteum-derived progenitor cells using immunophenotypes for chondrogenesis
SM Lim, YS Choi, HC Shin, CW Lee, DI Kim - Biotechnology letters, 2005 - Springer
SM Lim, YS Choi, HC Shin, CW Lee, DI Kim
Biotechnology letters, 2005•SpringerPeriosteum-derived progenitor cells (PDPCs) were isolated by characteristic surface
markers. Reproducibility of immunophenotypes of the PDPCs was characterized by flow
cytometric analysis using fluorescence-activated cell sorter (FACS). SH2+, SH3+, SH4+,
CD9+, CD90+ and CD105+ were important eternal characteristic cell surface markers for the
PDPCs. The characterized PDPCs maintained their chondrogenic potential in pellet cultures
until the 15th passage from primary cell culture.
markers. Reproducibility of immunophenotypes of the PDPCs was characterized by flow
cytometric analysis using fluorescence-activated cell sorter (FACS). SH2+, SH3+, SH4+,
CD9+, CD90+ and CD105+ were important eternal characteristic cell surface markers for the
PDPCs. The characterized PDPCs maintained their chondrogenic potential in pellet cultures
until the 15th passage from primary cell culture.
Abstract
Periosteum-derived progenitor cells (PDPCs) were isolated by characteristic surface markers. Reproducibility of immunophenotypes of the PDPCs was characterized by flow cytometric analysis using fluorescence-activated cell sorter (FACS). SH2+, SH3+, SH4+, CD9+, CD90+ and CD105+ were important eternal characteristic cell surface markers for the PDPCs. The characterized PDPCs maintained their chondrogenic potential in pellet cultures until the 15th passage from primary cell culture.
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