[HTML][HTML] Differential metabolomics reveals ophthalmic acid as an oxidative stress biomarker indicating hepatic glutathione consumption

T Soga, R Baran, M Suematsu, Y Ueno, S Ikeda… - Journal of Biological …, 2006 - ASBMB
Metabolomics is an emerging tool that can be used to gain insights into cellular and
physiological responses. Here we present a metabolome differential display method based
on capillary electrophoresis time-of-flight mass spectrometry to profile liver metabolites
following acetaminophen-induced hepatotoxicity. We globally detected 1,859 peaks in
mouse liver extracts and highlighted multiple changes in metabolite levels, including an
activation of the ophthalmate biosynthesis pathway. We confirmed that ophthalmate was …