Expression and purification of non-N-glycosylated porcine interleukin 3 in yeast Pichia pastoris

CE Hermanrud, V Pathiraja, A Matar… - Protein Expression and …, 2012 - Elsevier
Yeast Pichia pastoris has been widely utilized to express heterologous recombinant
proteins. P. pastoris expressed recombinant porcine interleukin 3 (IL3) has been used for
porcine stem cell mobilization in allo-hematopoietic cell transplantation models and pig-to-
primate xeno-hematopoietic cell transplantation models in our lab for many years. Since the
yeast glycosylation mechanism is not exactly the same as those of other mammalian cells, P.
pastoris expressed high-mannose glycoprotein porcine IL3 has been shown to result in a …