[HTML][HTML] Compensatory phosphorylation and protein-protein interactions revealed by loss of function and gain of function mutants of multiple serine phosphorylation …
PM Bauer, D Fulton, YC Boo, GP Sorescu… - Journal of biological …, 2003 - ASBMB
We examined the influence of individual serine phosphorylation sites in endothelial nitric-
oxide synthase (eNOS) on basal and stimulated NO release, cooperative phosphorylation,
and co-association with hsp90 and Akt. Mutation of the serine phosphorylation sites 116,
617, and 1179 to alanines affected the phospho-state of at least one other site,
demonstrating cooperation between multiple phosphorylation events, whereas mutation of
serine 635 to alanine did not cause compensation. Mutation of serines 116 and 617 to …
oxide synthase (eNOS) on basal and stimulated NO release, cooperative phosphorylation,
and co-association with hsp90 and Akt. Mutation of the serine phosphorylation sites 116,
617, and 1179 to alanines affected the phospho-state of at least one other site,
demonstrating cooperation between multiple phosphorylation events, whereas mutation of
serine 635 to alanine did not cause compensation. Mutation of serines 116 and 617 to …