[HTML][HTML] Short chain fatty acids (SCFA) reprogram gene expression in human malignant epithelial and lymphoid cells

L Astakhova, M Ngara, O Babich, A Prosekov… - PLoS …, 2016 - journals.plos.org
L Astakhova, M Ngara, O Babich, A Prosekov, L Asyakina, L Dyshlyuk, T Midtvedt, X Zhou…
PLoS One, 2016journals.plos.org
The effect of short chain fatty acids (SCFAs) on gene expression in human, malignant cell
lines was investigated, with a focus on signaling pathways. The commensal microbial flora
produce high levels of SCFAs with established physiologic effects in humans. The most
abundant SCFA metabolite in the human microflora is n-butyric acid. It is well known to
activate endogenous latent Epstein-Barr virus (EBV), that was used as a reference read out
system and extended to EBV+ epithelial cancer cell lines. N-butyric acid and its salt induced …
The effect of short chain fatty acids (SCFAs) on gene expression in human, malignant cell lines was investigated, with a focus on signaling pathways. The commensal microbial flora produce high levels of SCFAs with established physiologic effects in humans. The most abundant SCFA metabolite in the human microflora is n-butyric acid. It is well known to activate endogenous latent Epstein-Barr virus (EBV), that was used as a reference read out system and extended to EBV+ epithelial cancer cell lines. N-butyric acid and its salt induced inflammatory and apoptotic responses in tumor cells of epithelial and lymphoid origin. Epithelial cell migration was inhibited. The n-butyric gene activation was reduced by knock-down of the cell membrane transporters MCT-1 and -4 by siRNA. N-butyric acid show biologically significant effects on several important cellular functions, also with relevance for tumor cell phenotype.
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