[HTML][HTML] Cardiolipin-dependent reconstitution of respiratory supercomplexes from purified Saccharomyces cerevisiae complexes III and IV
S Bazán, E Mileykovskaya, VKPS Mallampalli… - Journal of Biological …, 2013 - ASBMB
Here, we report for the first time in vitro reconstitution of the respiratory supercomplexes from
individual complexes III and IV. Complexes III and IV were purified from Saccharomyces
cerevisiae mitochondria. Complex III contained eight molecules of cardiolipin, and complex
IV contained two molecules of cardiolipin, as determined by electrospray ionization-mass
spectrometry. Complex IV also contained Rcf1p. No supercomplexes were formed upon
mixing of the purified complexes, and low amounts of the supercomplex trimer III 2 IV 1 were …
individual complexes III and IV. Complexes III and IV were purified from Saccharomyces
cerevisiae mitochondria. Complex III contained eight molecules of cardiolipin, and complex
IV contained two molecules of cardiolipin, as determined by electrospray ionization-mass
spectrometry. Complex IV also contained Rcf1p. No supercomplexes were formed upon
mixing of the purified complexes, and low amounts of the supercomplex trimer III 2 IV 1 were …