Glucose stimulation of insulin secretion from the isolated pancreas of foetal and newborn rats

K Asplound, S Westman, C Hellersteöm - Diabetologia, 1969 - Springer
K Asplound, S Westman, C Hellersteöm
Diabetologia, 1969Springer
Pancreatic glands of 22-day-old rat foetuses or 0–12 day old rats were incubated in a Krebs-
Ringer phosphate-buffered salt solution, and the insulin secretion measured at a lower (0.6
mg/ml) or a higher (3 mg/ml) glucose concentration in the medium. Stimulation of the insulin
release by glucose could not be detected until the second postnatal day, when there was a
marked increase in the amount of hormone secreted into the medium. Since the presence of
insulin has been demonstrated in the B-cells of rat foetuses, the data suggest that in this …
Summary
Pancreatic glands of 22-day-old rat foetuses or 0–12 day old rats were incubated in a Krebs-Ringer phosphate-buffered salt solution, and the insulin secretion measured at a lower (0.6 mg/ml) or a higher (3 mg/ml) glucose concentration in the medium. Stimulation of the insulin release by glucose could not be detected until the second postnatal day, when there was a marked increase in the amount of hormone secreted into the medium. Since the presence of insulin has been demonstrated in the B-cells of rat foetuses, the data suggest that in this species the cellular mechanism for insulin synthesis becomes manifest considerably earlier than that for glucose-regulated insulin release.
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