Purpose To investigate the induction of type III interferons (IFNs) in human cancer cells by
gamma-rays. Methods and Materials Type III IFN expression in human cancer cell lines after
gamma-ray irradiation in vitro was assessed by reverse transcription–quantitative
polymerase chain reaction and enzyme-linked immunosorbent assay. Signaling pathways
mediating type III IFN induction were examined by a variety of means, including
immunoblotting, flow cytometry, confocal imaging, and reverse transcription–quantitative …