Defining blood processing parameters for optimal detection of cryopreserved antigen-specific responses for HIV vaccine trials
M Bull, D Lee, J Stucky, YL Chiu, A Rubin… - Journal of …, 2007 - Elsevier
Interferon-gamma (IFN-γ) ELISpot and intracellular cytokine staining (ICS) assays are
routinely employed in clinical HIV vaccine trials to identify antigen-specific T cells in
cryopreserved peripheral blood mononuclear cells (PBMC). Several parameters involved in
blood collection, processing and shipping may influence immunological function of the
resulting cells, including anticoagulant type, time from venipuncture to PBMC isolation/
cryopreservation, method of PBMC isolation and procedure for sample shipping. We …
routinely employed in clinical HIV vaccine trials to identify antigen-specific T cells in
cryopreserved peripheral blood mononuclear cells (PBMC). Several parameters involved in
blood collection, processing and shipping may influence immunological function of the
resulting cells, including anticoagulant type, time from venipuncture to PBMC isolation/
cryopreservation, method of PBMC isolation and procedure for sample shipping. We …