[HTML][HTML] A scalable, fully automated process for construction of sequence-ready human exome targeted capture libraries

S Fisher, A Barry, J Abreu, B Minie, J Nolan… - Genome biology, 2011 - Springer
S Fisher, A Barry, J Abreu, B Minie, J Nolan, TM Delorey, G Young, TJ Fennell, A Allen…
Genome biology, 2011Springer
Genome targeting methods enable cost-effective capture of specific subsets of the genome
for sequencing. We present here an automated, highly scalable method for carrying out the
Solution Hybrid Selection capture approach that provides a dramatic increase in scale and
throughput of sequence-ready libraries produced. Significant process improvements and a
series of in-process quality control checkpoints are also added. These process
improvements can also be used in a manual version of the protocol.
Abstract
Genome targeting methods enable cost-effective capture of specific subsets of the genome for sequencing. We present here an automated, highly scalable method for carrying out the Solution Hybrid Selection capture approach that provides a dramatic increase in scale and throughput of sequence-ready libraries produced. Significant process improvements and a series of in-process quality control checkpoints are also added. These process improvements can also be used in a manual version of the protocol.
Springer