[HTML][HTML] Vinblastine-induced phosphorylation of Bcl-2 and Bcl-XL is mediated by JNK and occurs in parallel with inactivation of the Raf-1/MEK/ERK cascade
M Fan, M Goodwin, T Vu, C Brantley-Finley… - Journal of Biological …, 2000 - ASBMB
Microtubule-damaging agents arrest cells at G 2/M and induce apoptosis in association with
phosphorylation of the anti-apoptotic proteins Bcl-2 and Bcl-X L. Because microtubule
inhibitors activate JNK, we sought to determine whether JNK was responsible for Bcl-2/Bcl-X
L phosphorylation in KB-3 cells treated with vinblastine. Two major endogenous forms of
JNK, p46 JNK1 and p54 JNK2, were present in KB-3 cells, and both isoforms were activated
by vinblastine as determined by Mono Q chromatography. We used antisense …
phosphorylation of the anti-apoptotic proteins Bcl-2 and Bcl-X L. Because microtubule
inhibitors activate JNK, we sought to determine whether JNK was responsible for Bcl-2/Bcl-X
L phosphorylation in KB-3 cells treated with vinblastine. Two major endogenous forms of
JNK, p46 JNK1 and p54 JNK2, were present in KB-3 cells, and both isoforms were activated
by vinblastine as determined by Mono Q chromatography. We used antisense …