[HTML][HTML] Free, unlinked glycosylphosphatidylinositols on mammalian cell surfaces revisited

Y Wang, T Hirata, Y Maeda, Y Murakami… - Journal of Biological …, 2019 - ASBMB
Glycosylphosphatidylinositols (GPIs) are linked to many cell-surface proteins, anchor these
proteins in the membrane, and are well characterized. However, GPIs that exist in the free
form on the mammalian cell surface remain largely unexplored. To investigate free GPIs in
cultured cell lines and mouse tissues, here we used the T5-4E10 mAb (T5 mAb), which
recognizes unlinked GPIs having an N-acetylgalactosamine (GalNAc) side chain linked to
the first mannose at the nonreducing terminus. We detected free GPIs bearing the GalNAc …