[HTML][HTML] Biochemical evidence for the involvement of tyrosine in epoxide activation during the catalytic cycle of epoxide hydrolase
T Yamada, C Morisseau, JE Maxwell… - Journal of Biological …, 2000 - ASBMB
Epoxide hydrolases (EH) catalyze the hydrolysis of epoxides and arene oxides to their
corresponding diols. The crystal structure of murine soluble EH suggests that Tyr 465 and
Tyr 381 act as acid catalysts, activating the epoxide ring and facilitating the formation of a
covalent intermediate between the epoxide and the enzyme. To explore the role of these two
residues, mutant enzymes were produced and the mechanism of action was analyzed.
Enzyme assays on a series of substrates confirm that both Tyr 465 and Tyr 381 are required …
corresponding diols. The crystal structure of murine soluble EH suggests that Tyr 465 and
Tyr 381 act as acid catalysts, activating the epoxide ring and facilitating the formation of a
covalent intermediate between the epoxide and the enzyme. To explore the role of these two
residues, mutant enzymes were produced and the mechanism of action was analyzed.
Enzyme assays on a series of substrates confirm that both Tyr 465 and Tyr 381 are required …