Establishment of four functional, clonal strains of animal cells in culture

Y Yasumura, AH Tashjian Jr, GH Sato - Science, 1966 - science.org
Y Yasumura, AH Tashjian Jr, GH Sato
Science, 1966science.org
The single-cell plating technique was used to develop four clonal cell lines that perform
organ-specific functions after being serially cultured for prolonged periods. These strains
include steroid-secreting Leydig cells, melanoma cells that form pigment, and two strains
from a hormone-secreting rat pituitary tumor. One of the cell lines from the pituitary tumor
secretes growth hormone, while another line derived from the same tumor secretes a
substance similar to adrenocorticotropic hormone.
The single-cell plating technique was used to develop four clonal cell lines that perform organ-specific functions after being serially cultured for prolonged periods. These strains include steroid-secreting Leydig cells, melanoma cells that form pigment, and two strains from a hormone-secreting rat pituitary tumor. One of the cell lines from the pituitary tumor secretes growth hormone, while another line derived from the same tumor secretes a substance similar to adrenocorticotropic hormone.
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