Establishment of a continuous tumor‐cell line (PANC‐1) from a human carcinoma of the exocrine pancreas
M Lieber, J Mazzetta, W Nelson‐Rees… - … journal of cancer, 1975 - Wiley Online Library
M Lieber, J Mazzetta, W Nelson‐Rees, M Kaplan, G Todaro
International journal of cancer, 1975•Wiley Online LibraryAn epithelioid cell line, started from a human pancreatic carcinoma of ductal cell origin, has
been maintained in culture for over 2 years and has been subcultured more than 40 times.
The PANC‐1 cell line has a doubling time of 52 h and G6PD activity of the slow mobility or B
type. Chromosome studies show a modal number of 63 with three distinct marker
chromosomes and a small ring chromosome. The malignant nature of the PANC‐1 cell line
was verified by:(1) the ready growth of PANC‐1 cells in soft agar and on top of a fibroblast …
been maintained in culture for over 2 years and has been subcultured more than 40 times.
The PANC‐1 cell line has a doubling time of 52 h and G6PD activity of the slow mobility or B
type. Chromosome studies show a modal number of 63 with three distinct marker
chromosomes and a small ring chromosome. The malignant nature of the PANC‐1 cell line
was verified by:(1) the ready growth of PANC‐1 cells in soft agar and on top of a fibroblast …
Abstract
An epithelioid cell line, started from a human pancreatic carcinoma of ductal cell origin, has been maintained in culture for over 2 years and has been subcultured more than 40 times. The PANC‐1 cell line has a doubling time of 52 h and G6PD activity of the slow mobility or B type. Chromosome studies show a modal number of 63 with three distinct marker chromosomes and a small ring chromosome. The malignant nature of the PANC‐1 cell line was verified by: (1) the ready growth of PANC‐1 cells in soft agar and on top of a fibroblast monolayer; and (2) the formation of a progressively growing anaplastic carcinoma after injection of a nude‐athymic mouse with PANC‐1 cells.
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