Robust 4C-seq data analysis to screen for regulatory DNA interactions
HJG Van De Werken, G Landan, SJB Holwerda… - Nature …, 2012 - nature.com
HJG Van De Werken, G Landan, SJB Holwerda, M Hoichman, P Klous, R Chachik…
Nature methods, 2012•nature.comRegulatory DNA elements can control the expression of distant genes via physical
interactions. Here we present a cost-effective methodology and computational analysis
pipeline for robust characterization of the physical organization around selected promoters
and other functional elements using chromosome conformation capture combined with high-
throughput sequencing (4C-seq). Our approach can be multiplexed and routinely integrated
with other functional genomics assays to facilitate physical characterization of gene …
interactions. Here we present a cost-effective methodology and computational analysis
pipeline for robust characterization of the physical organization around selected promoters
and other functional elements using chromosome conformation capture combined with high-
throughput sequencing (4C-seq). Our approach can be multiplexed and routinely integrated
with other functional genomics assays to facilitate physical characterization of gene …
Abstract
Regulatory DNA elements can control the expression of distant genes via physical interactions. Here we present a cost-effective methodology and computational analysis pipeline for robust characterization of the physical organization around selected promoters and other functional elements using chromosome conformation capture combined with high-throughput sequencing (4C-seq). Our approach can be multiplexed and routinely integrated with other functional genomics assays to facilitate physical characterization of gene regulation.
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