[HTML][HTML] The p63 gene is regulated by grainyhead-like 2 (GRHL2) through reciprocal feedback and determines the epithelial phenotype in human keratinocytes
S Mehrazarin, W Chen, JE Oh, ZX Liu, KL Kang… - Journal of Biological …, 2015 - ASBMB
In this study, we investigated the effects of p63 modulation in epithelial plasticity in human
keratinocytes. The p63 isoforms ΔNp63α, ΔNp63β, and ΔNp63γ were ectopically expressed
in normal human epidermal keratinocytes (NHEKs). The epithelial or mesenchymal state
was determined by morphological changes and altered expression of various markers, eg
fibronectin, E-Cadherin, and keratin 14. Overexpression of ΔNp63α and ΔNp63β but not
ΔNp63γ isoforms led to morphological changes consistent with epithelial-mesenchymal …
keratinocytes. The p63 isoforms ΔNp63α, ΔNp63β, and ΔNp63γ were ectopically expressed
in normal human epidermal keratinocytes (NHEKs). The epithelial or mesenchymal state
was determined by morphological changes and altered expression of various markers, eg
fibronectin, E-Cadherin, and keratin 14. Overexpression of ΔNp63α and ΔNp63β but not
ΔNp63γ isoforms led to morphological changes consistent with epithelial-mesenchymal …