[HTML][HTML] Standard operating procedure for somatic variant refinement of sequencing data with paired tumor and normal samples
EK Barnell, P Ronning, KM Campbell, K Krysiak… - 2019 - Elsevier
Purpose Following automated variant calling, manual review of aligned read sequences is
required to identify a high-quality list of somatic variants. Despite widespread use in
analyzing sequence data, methods to standardize manual review have not been described,
resulting in high inter-and intralab variability. Methods This manual review standard
operating procedure (SOP) consists of methods to annotate variants with four different calls
and 19 tags. The calls indicate a reviewer's confidence in each variant and the tags indicate …
required to identify a high-quality list of somatic variants. Despite widespread use in
analyzing sequence data, methods to standardize manual review have not been described,
resulting in high inter-and intralab variability. Methods This manual review standard
operating procedure (SOP) consists of methods to annotate variants with four different calls
and 19 tags. The calls indicate a reviewer's confidence in each variant and the tags indicate …
