Phosphate-binding tag, a new tool to visualize phosphorylated proteins
E Kinoshita, E Kinoshita-Kikuta, K Takiyama… - Molecular & cellular …, 2006 - ASBMB
We introduce two methods for the visualization of phosphorylated proteins using alkoxide-
bridged dinuclear metal (ie Zn 2+ or Mn 2+) complexes as novel phosphate-binding tag
(Phos-tag) molecules. Both Zn 2+-and Mn 2+-Phos-tag molecules preferentially capture
phosphomonoester dianions bound to Ser, Thr, and Tyr residues. One method is based on
an ECL system using biotin-pendant Zn 2+-Phos-tag and horseradish peroxidase-
conjugated streptavidin. We demonstrate the electroblotting analyses of protein …
bridged dinuclear metal (ie Zn 2+ or Mn 2+) complexes as novel phosphate-binding tag
(Phos-tag) molecules. Both Zn 2+-and Mn 2+-Phos-tag molecules preferentially capture
phosphomonoester dianions bound to Ser, Thr, and Tyr residues. One method is based on
an ECL system using biotin-pendant Zn 2+-Phos-tag and horseradish peroxidase-
conjugated streptavidin. We demonstrate the electroblotting analyses of protein …
