Using enhanced Green fluorescence protein-expressing Escherichia Coli to assess mouse peritoneal macrophage phagocytosis
Y Zhang, G Wang, J Lu, L Xu, J Xiong - JoVE (Journal of Visualized …, 2019 - jove.com
This manuscript describes a simple and reproducible method to perform a phagocytosis
assay. The first part of this method involves building a pET-SUMO-EGFP vector (SUMO=
small ubiquitin-like modifier) and expressing enhanced green fluorescence protein (EGFP)
in Escherichia coli (BL21DE). EGFP-expressing E. coli is coincubated with macrophages for
1 h at 37° C; the negative control group is incubated on ice for the same amount of time.
Then, the macrophages are ready for assessment. The advantages of this technique include …
assay. The first part of this method involves building a pET-SUMO-EGFP vector (SUMO=
small ubiquitin-like modifier) and expressing enhanced green fluorescence protein (EGFP)
in Escherichia coli (BL21DE). EGFP-expressing E. coli is coincubated with macrophages for
1 h at 37° C; the negative control group is incubated on ice for the same amount of time.
Then, the macrophages are ready for assessment. The advantages of this technique include …