Immunofluorescence microscopy of metabolic enzymes. Representative neutrophils from nonpregnant women (first and second columns) and pregnant women (third and fourth columns) are shown. Neutrophils were stained with and without prior incubation with LPS (50 ng/ml, 20 minutes). Cells were stained with antibodies directed against hexokinase (a–d), G-6-PDase (e–h), PFK (i–l), PK (m–p), and LDH (q–t). Hexokinase translocates to the periphery of stimulated neutrophils from nonpregnant and pregnant subjects (b and d). G-6-PDase is peripherally located under all conditions in cells from nonpregnant women but was found to be uniform or concentrated near the centrosome in maternal cells. Thus, G-6-PDase undergoes differential trafficking in cells from nonpregnant and pregnant women. Although PK undergoes significant trafficking in morphologically polarized neutrophils, no distinction between control and pregnancy neutrophils was noted. n = 16. ×480.